Coding

Part:BBa_J34808:Design

Designed by: Alexandra Choutko   Group: iGEM2006_ETHZ   (2006-10-26)

TEV protease


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 385
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 385
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 476
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 385
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 385
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 319
    Illegal SapI.rc site found at 667


Design Notes

Source

References

Sreedevi Nallamsetty et al., Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro, Protein Expression and Purification 38 (2004) 108–115 Rachel B. Kapust and David S. Waughn, Controlled Intracellular Processing of Fusion Proteins by TEV Protease, Protein Expression and Purification 19, 312–318 (2000)